Journal: bioRxiv
Article Title: Hox – Meis -relayed topographical genetic switch underlies cardiopharyngeal neural crest diversification, revealed by multimodal analysis
doi: 10.1101/2025.11.09.687497
Figure Lengend Snippet: (a) Scheme of GRN analysis using Fluidigm C1 scRNA-seq data. Dashed-line boxes indicate the TF subnetwork, which was divided into overlapping communities. Each TF is connected to its first-edge targets to define “overall communities” (OCs). (b) Heatmap showing the enrichment of representative OCs across integrated clusters. Color scale represents z-scored enrichment values. (c) UMAP plots showing representative OC enrichment patterns, colored by cluster identity as in (d) with intensity indicating enrichment levels. (d) Schematic representation of NCC lineage trajectories annotated with OCs and key TFs.
Article Snippet: Single-cell cDNAs were prepared using SMART-Seq v4 Ultra Input Low RNA kit for the Fluidigm C1 System (Clontech), and quality was confirmed with the Agilent 2200 TapeStation and quantified by Qubit. (Thermo Fisher).
Techniques: